HiFi DNA Polymerase contains TA DNA Polymerase and a proofreading 3’-5’ exonuclease.HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TA DNA Polymerase. Two different buffers are provided in the kit. HiFi PCR Buffer I is optimized for the amplification of genomic DNA and HiFi PCR Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Extension rate is about 1-2 kb/min.
Template-independent “A” can be generated at the 3’ end of the PCR product.
Amplification of genomic DNA fragment up to 15 kb.
